Pure cultures of Tuber indicum, T. lijiangense, and T. elevatireticulatum were obtained through
isolation of fertile tissues of fruiting bodies in 1/2 Murashige and Skoog (MS) agar medium. Molecular
identification of the fruiting bodies and pure cultures were carried out. Tuber indicum was
subcultured on different types of agar medium and showed growth on 1/2 MS, yeast extract agar
(YEA), potato dextrose agar (PDA), YMTA, and soil extract agar medium. Tuber indicum and T.
elevatireticulatum exhibited the best growth on 1/2 MS medium, while T. lijiangense exhibited the
best growth on YMTA medium. Mastering isolation and culturing skills of Tuber spp. to obtain
mycelium culture can assist investigations into mutualistic relationships between Tuber spp. and
different host trees, and also be applied to truffle production in the future.