Abstract
Production of Antrodia camphorata fruit in natural woods of Cinnamomum kanehirae has been developed in biotech companies in Taiwan. Since the natural trees of C. kanehirae are protected, woods from the man-made plantation become the only source. We found that C. kanehirae plants via tissue culture growing rapidly with greater survival than cutting plants. Therefore we try to develop a mass production system of tissue culture of C. kanehirae to increase multiplication rate and reduced production cost. We also try to build a platform from in vitro culture of A. camphorata, inoculation and fruit production on branches and logs of C. kanehirae logs harvest from different ages of man-made plantation. Nothapodytes nimmoniana trees, a major source of camptotheicn (CPT) that is a precursor to synthesize anticancer drug, have been created a great profit in biotech company in Taiwan. The processs of traditional management begins to plant trees for 5 yeas and harvest trunks to extract CPT. The branches are discarded. We selected some chemical races of N. nimmoniana that contains high concentration CPT and its analogues, and develop a sustainable management system by harvesting branches annually. To mass producing these few superior clones, tissue culture is the best step. Once more clonal plants are propagated in vitro, we will established a scion garden to produce cutting plants. Plantations established in these two sources will be compared in cost and efficiency. In farm management we will analyze the variation and concentrations of CPT analogus in fresh and different drying/powdering treated branches to find the best yield of both biomass and CPT analogus. This year, we have established the suitable combination between the numberes of in vitro culturs and transplanted plantlets of C. kanehirae. We can transplant plantlets 1,500 per 1.5 months, or 4,500 per 4.5 months, as modified for plantation seasons. We also identified the component of 9MCPT in N. nimmoniana leaves using UPLC-TOF. CPT and 9MCPT in fresh leaves of 27 clones from 5 provenances were analyzed. CPT concentrations ranged from 2000 to 4000 ppm, while 9MCPT from 900 to 2000 ppm.