Abstract
一、We have obtained hairy root lines of Taxus sumatrana, Nothopodytes nimmoniana, Camptotheca acuminata, and Cephalotaxus wilsoniana which transformed with rol gene. These root lines grew rapidly and produced stably secondary metabolites greater and more easily to preserve than that from cell cultures. The limitation is that they are difficult to scale up in batch cultures. This study is trying to improve culture techniques including to enhance transformation rates of hairy roots, to increase biomass coupling with high concentration of secondary metabolites, to design suitable bioreactors to grow root cultures, and to establish a cooperative platform among research, application, and biotech companies. 二、For developing the forestry genomic research, the nucleotide sequencing plays the important role. In this project, the specific tree species’ genome and transcriptome will be sequencing by using next generation sequencing. The sequencing data will provide useful information for developing molecular marker and improve the studies of the population genetics, molecular breeding and species identification. In addition, this project will manage and preserve the valuable forestry genomic germplasm resources which we had (or the species with highly application potential) in Germplasm Bank. According to characters of superior tree varieties, we will develop preservation methods in vitro to prevent loss of specific genes. We also will provide the strategy of value added regulations for conservation, storage and application of these genomic germplasm.