Abstract
The aim of this project is a collaborative research in coastal tree research between Taiwan Forestry Research Institute (TFRI) and Forestry and Forest Products Research Institute (FFPRI) in Japan. The first coastal tree we select is Calophyllum inophyllum. The first objectives is to select wind tolerant plus trees among Taiwan, Iriomote island and Ishigaki island, and to collect seeds and cultivate seedlings. These seedlings will be planted at coastal area, and seedlings survival and growth will be investigated. These data with growth difference and wind tolerance will be determined. The second objective is to analyze genetic variation among Hernandia nymphiifolia populations among Taiwan, Iriomote island and Ishigaki island. The analyzing data will include genetic diversity within populations (islands), genetic differentiation and phylogeography among populations (islands). Total of 37 Calophyllum inophyllum plus trees have been investigated in Taiwan, including Hengchung 21 plus trees, Hualien 4 plus trees and Taitung 12 plus trees. Each plus tree with about 100 seeds had been collected, and total 1,323 seedlings have been obtained. Total of 20 plus trees had been investigated in Okinawa, and 480 seedlings were obtained. In 2016, Taiwan seedlings of 32 plus trees and Japan 19 plus trees 951 seedlings are also have been planted at Hengchun and Illan sited.In this study, the genetic variation and population structure of 76 individuals sampled from 8 populations of Hernandia nymphiifoliain Taiwan, Green Island Lanyu and Japan were studied using ISSR markers. POPGENE analysis revealed that total gene diversity (H) was 0.1790 and genetic differentiation index (Gst) was 0.6838, while gene flow index (Nm) was 0.1156, which indicated that the genetic differentiation was high among populations. In addition, Mantel test showed there was no significant relation between genetic distance and geographic distance (r=0.83, p=0.99), pointing to a random distribution among populations. Based on the above finding, both In-situ and Ex-situ conservation are necessary in preserving this species.