執行成果摘要
Root and basal stem rot diseases are not easy to be found at the initial infection stage and some latent infection also develop, which cause the difficulty of disease control and may spread out the pathogen unintentionally by afforestation or transplantation. The project purpose in this year is to establish rapid molecular detection technology for Ganoderma root and butt rot. The pure cultures and preservations of Ganoderma austral, G. applanatum, G. boninense, G. tropicum and G. weberianum are accomplished. The five fungal DNA are subjected to PCR reaction with the common primer set, ITS4/ITS5, and the products are sequenced respectively. Then the conserve DNA fragment of G. austral are discovered by aid of alignment software to design 4 primer combinations. After specificity and sensitivity tests, 3 combinations showed good amplification results and can be applied in the examination and determination of Ganoderma root and butt rot in the future. Root and basal stem rot diseases are not easy to be found at the initial infection stage and some latent infection also develop, which cause the difficulty of disease control and may spread out the pathogen unintentionally by afforestation or transplantation. The project purpose in this year is to establish rapid molecular detection technology for Ganoderma root and butt rot. The pure cultures and preservations of Ganoderma austral, G. applanatum, G. boninense, G. tropicum and G. weberianum are accomplished. The five fungal DNA are subjected to PCR reaction with the common primer set, ITS4/ITS5, and the products are sequenced respectively. Then the conserve DNA fragment of G. austral are discovered by aid of alignment software to design 4 primer combinations. After specificity and sensitivity tests, 3 combinations showed good amplification results and can be applied in the examination and determination of Ganoderma root and butt rot in the future.