執行成果摘要
In this study, Melaleuca (Melaleuca leucadendra) and Beech (Zelkova serrata) MeOH and EtOH crude extracts of different concentrations of solvent extraction and hot water extraction , crude extract by liquid - liquid distribution into Hexane, Ethyl acetate, 4 soluble fraction Butanol and Water , etc. As for biological activity assessment includes DPPH radical scavenging ability , shrimp lethality test, the anti-inflammatory activity of the test , the test cell whitening , anti- rot test , anti- mosquito larvae test . Butanol soluble fraction was found Melaleuca branches, leaves with a small amount of EtOH extract of DPPH radical scavenging ability , Central Beech Leaves 60%, 95% EtOH extract crude extract , the inhibition rates were IC50 = 33.21 ± 0.84 μg / mL , IC50 = 75.82 ± 1.44 μg / mL, clear inhibition of DPPH radical better rate , followed by the different soluble in beech leaves 95% EtOH extract crude extract , northern, central, Nanzhi leaf extract crude extract of EtOH Ministry of different soluble portion of the branch 's begin with Ethyl acetate EtOH extract the soluble portion , different soluble portion Butanol extraction of the soluble portion of the leaf EtOH have better scavenging DPPH radicals . The shrimp lethality test results showed that Melaleuca oil has significant toxic effects , but leaves EtOH extract crude extract at 48 and 72 hours is not much for shrimp toxicity , beech crude extract with shrimp toxicity test increase the time and crude extract and different concentrations of the soluble portion , shrimp mortality increased. Melaleuca oil and tea tree oil compared to Australia , particularly in the concentration of 50 ppm oil Melaleuca better , able to inhibit LPS -induced RAW 264.7 murine macrophage nitric oxide generated by one (NO), which has a good display the anti-inflammatory activity. Inhibition of tyrosinase activity results in the cells showed , there are 50% -80 % of the inhibitory effect on melanin content in cells showed that the higher the concentration , the better the effect of melanin , Kojin acid comparison with the control group , both with 55% the inhibition of melanin synthesis results. Anti- Aedes albopictus larvae test results show that essential oils can be completely inhibited larvae activity , antifungal test results show that Melaleuca , beech and crude extracts and essential oils of different soluble portion , from low concentration to high concentration , both for Trametes versicolo white rot fungi , Gloeophyllum trabeum, Laetiporus sulphureus brown rot fungi and brown root rot fungus Phellinus noxius both have good antibacterial activity, integrated above test results show that the melaleuca leaf EtOH extract crude extract is not toxic to cells , does not have anti-inflammatory activity , but compared with Melaleuca oil , essential oils are having a good inhibition of intracellular tyrosinase activity , inhibiting cell melanin content , anti- rot, anti- mosquito and anti -inflammatory activity.