執行成果摘要
Magnolia genus was the most common active ingredient Magnolol and its isomers Honokiol, which had many internationally medicinal instances.Magnolia kachirachirae (guei tian , Shou ka area ) , Manglietiastrum sinicum and Liriodendron chinense crude extract of the leaves obtained by methanol, ethanol extraction. Manglietiastrum sinicum MeOH leaf extract is three Magnolia genus best antioxidants. Magnolia kachirachirae leaf extract crude extracts of different parts of MeOH soluble portion , Butanol soluble fraction parts (IC50 = 88.45 ± 2.93 μg / mL) had better scavenging DPPH radicals. Shrimp lethality test, in addition to MKG ( Country Life area ) leaves powder of EtOH crude extract , with significant cytotoxicity , the rest are non -toxic too. Magnolia kachirachirae essential oil has increased cell survival rate compared with β-Caryophyllene and Caryophyllene oxide. Those were highly resistant to inflammation and cell survival, Hexane soluble fraction and Ethyl acetate soluble portion had the cells cytotoxicity with high concentrations. Inhibition of tyrosinase activity in cells results show Kojic acid comparison with the control group , in addition to MKS (Shou ka area ) 95% EtOH and 60% EtOH crude extract of the poor activity , the remaining extract also has 65% -80 % the inhibitory effect and the ability to inhibit the synthesis of melanin. Crude extracts and essential oils from Magnolia kachirachirae methanol extract of the leaves of the proinflammatory cytokine response , with a significant inhibition of the IL-1β production, reduced the generation of proinflammatory cytokines .