執行成果摘要
There are more than 380 species of wild orchids in Taiwan, accounting for about 1/10 of Taiwan's vascular plants, and reveal high importance for forest biodiversity. However, the populations of wild orchids were severely damaged especially those in the low altitude because of the fast development and rapid change of terrain habitats in low altitude of Taiwan. Thus, many exiguous orchids have been largely removed or are on the brink of extinction due to the loss of their original habitats. In addition to the simple of being cultivated in city, the beautiful orchids in low altitude always become targets for commercial collection.
It is necessary to engage in the in-situ and ex-situ conservation to avoid these extincting wild orchids disappear from Taiwan by human factors. This project engaged in the ecological survey of those exiguous orchids listed in the plant red book, and collected their seeds for various breeding research.
The main results of this year are as follows:
（1）Asymbiotic seed germination and seedling production of Vanda lamellata :
The mature fruits were collected in Hsiaolanyu. One month later after sowed, the protocom were developed, then numerous absorption root hairs and leaves were growth in about two months. After subculturing, the roots were growth successfully.
（2）Asymbiotic seed germination of Dendrobium miyakei with six different mediums:
The plants Dendrobium miyakei were collected in Lanyu. Asymbiotic seed germination in mediums of PA2、PA1、PA4、VW、1/2VW and HYPONeX, the germination rate in VW is the highest (95.19%), and in HYPONeX is the lowest (65.10%).
（3）Germination of Spathoglottis plicata by PA1 medium:
Spathoglottis plicata were collected on July 1 in Green Island. Planting the seeds into PA1 medium, and subculturing in September and November.
（4）Pedicel culture of Phalaenopsis equestris:
The first culture of pedicel of Phalaenopsis equestris was finished on September 5. One apical bud was separated to 4 buds, and one of them was contaminated. We used the bud nodes to do slice culture for two months, the apical buds did not brown but not grow, and the nodes did not grow either.
（5）Pedicel culture of Phalaenopsis aphrodite subsp. formosana:
The fruits were collected in Manjhou native habitat, the first culture of pedicel were done on August 2, using 2 apical buds to separated to 18 buds, 7 buds were contaminated. The culture are proceeded about three months, 3 buds are growing, and the others were browning. Every six weeks subculture once.