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DNA barcode and the preservation and utilization of genetic resources of the forestry species in Tai

  • Date of declaration:2012-06-11
  • PI:Wen-Liang Chiou
  • Division:Botanical Garden Division
Research title
Science and Technology Programs(2010)
Abstract
The DNA barcode data of Taiwan’s native gymnosperms established using cpDNA non-coding region showed that the lengths of petG-trnP, trnL-trnF and trnV-trnM were 326-447bp, 247-489bp and 732-857bp respectively, along with 106 complete sequences collected on 2009. Through genetic analysis of the three cpDNA fragments, analytical capacity can reach the genus.
Population genetic and phylogeography studies on Cyclobalanopsis pachyloma in Taiwan used the nucleic fragment of CRC gene. We have completed all 199 samples of CRC gene fragments, sequence length between 931 bp to 962 bp, with a large number of point mutations. Neighbor-joining tree showed branch clustering phenomenon.
We investigated three sampling plots in the forest. Because of differences in altitude and forest forms, the species of wood decay fungi have their differences. There have similar wood-decay fungus on Wushibi Natural Coast Reserving Area and Hualian Shakadang Trail, by the types of tropics and subtropics primarily, such as Coriolopsis aspera, C. neaniscus, Hexagonia tenuis, Ganoderma australe, G. tropicum, Microporus affinis, Stereum ostrea and Echinochaete russiceps. Wood-decay fungus in Taiwan Beech Forest Trail is mainly on the temperate broadleaf forest and coniferous forest types, such as Ganoderma australe, Trametes versicola, T. hirsuta, Microporus affinis, Stereum ostrea, Fomitopsis pinicola, Oudemansiella mucida and Lampteromyces japonicus. Besides, we found that Ganoderma australe, Monoporeia affinis and Stereum ostrea were the most common wood decay fungi investigated in this three sampling plots judging from the data of more than 40 strains separation.