執行成果摘要
一、Applications of DNA marker in forestry tree species:
In this study, through the development of DNA markers, including SSR, SNP, Indel, etc., to explore the genetic resources of valuable forest trees. At the same time we are maintaining, constructing, and conserving valuable forest tree provenance areas, production and cultivation areas, nurseries, and more expect that through the genomics researches in forest to develop useful DNA markers to discriminate and heredity utility.二、The conservation and application of the genomics and production of secondary products in forestry:
We have obtained hairy root lines of Taxus sumatrana, Nothopodytes nimmoniana, Camptotheca acuminata, and Cephalotaxus wilsoniana which transformed with rol gene. These root lines grew rapidly and produced stably secondary metabolites greater and more easily to preserve than that from cell cultures. The limitation is that they are difficult to scale up in batch cultures. This study is trying to improve culture techniques including to enhance transformation rates of hairy roots, to increase biomass coupling with high concentration of secondary metabolites, to design suitable bioreactors to grow root cultures, and to establish a cooperative platform among research, application, and biotech companies.三、Molecular phylogeny of Dianthus and its resource conservation and utilization:
The genus Dianthus in Taiwan includes Dianthus palinensis , Dianthus pygmaeus , Dianthus seisuimontanus , Dianthus superbus var. longicalycinus, Dianthus superbus var. Each of which has ornamental value and useness potential. At present, there are also many kinds of plants that have become horticultural plants, but Dianthus in Taiwan still have many chaos in morphology, and there are still many controversies in classification and identification. It is urgent to explore their genetic variation and relatives at the molecular level. The research results can be used as a reference for this genus in resource utilization and conservation.四、The application of microbial genetic analysis in Calophyllum inophyllum:
Since Calophyllum inophyllum adult trees have high tolerance to abiotic stress in their habitat, this study is planned to isolate and screen potential plant growth-promoting bacteria (PGPB) from roots of native Calophyllum inophyllum trees, with the aim of applying them to enhance the survival and growth of Calophyllum inophyllum and other tree seedlings in drought and saline environments. Besides, for benefiting further investigation of the effects of inoculation of potential PGPB strains on the host plants and their rhizosphere, soil properties and microbial community of the collected Calophyllum inophyllum rhizosphere and soil samples are planned to be analyzed in this study. It is expected that this study will lead to the development of beneficial bacteria with application value on afforestation sites and a better understanding of the biotic and abiotic nature of the environment from which the PGPB strains originate.