公告日期 : 2014-12-30
Application of Biocontrol Agents to Control Phellinus noxius
Authors：Meng-Ling Wu, Yu-Yen Hsu, Chih-Yun Lee,Hew-Der Wu, Ting-Hsuan Hung
Key words：brown root rot disease, Phellinus noxius, microbiology, biocontrol agent, polymerasechain reaction (PCR)
In this study, biocontrol agents, derived from 4 screened microorganisms, for preventing
the growth of Phellinus noxius which causes brown root rot disease were tested using
simulations and field tests. The technology of a polymerase chain reaction (PCR) specific
for P. noxius, created in our laboratory, was used to monitor the control efficiency of the
biocontrol agents against P. noxius infection. Phellinus noxius covered the entire PDA
culture dish (at 9 cm in diameter) within 4 d in the control group; in contrast diameters of
colonies ranged 0.62~7.8 cm in the test groups bearing the 4 screened biocontrol agents.
These results revealed that all agents possessed control potential against P. noxius
infection. Of these biocontrol agents, 2 showed the best control efficiency and persistency.
The P. noxius stubs in moistened soil for 7 wk retained about 88.89 and 80% germ
survival on cassia and banyan stubs in the simulated field test, respectively. Interestingly,
P. noxius was inhibited when the soil was moistened with 0.5x, 1x, and 10x of biocontrol
agent 2. In the actual field tests, we found that 1 Philippine ebony persimmon infected by P.
noxius gradually grew a green leaf from the withered portion after treatment with biocontrol
agent 2 for 8 wk, consistent with a decrease in the P. noxius population detected by the
PCR. Likewise, detectable P. noxius was gradually reduced with treatment time with
biocontrol agent 2 from Taiwanese and 20 flameinfected trees. Collectively, damage by P.
noxius can be significantly ameliorated by the biocontrol agents examined in this study.