Authors：Liao YK, Chuang MC.

Year：2014

Key words：activated charcoal, micropropagation, Quercus aliena Blume var. aliena, silver nitrate.

　This study established a micropropagation method for the tissue culture propagation of mature Quercus aliena Blume var. aliena. The method was modified from procedures successfully applied to other species of the same genus, and it was tested for its efficiency on mature explants of the target species. Stem nodal segments collected from trunk sprouts were used as the original explants. The newly induced axillary shoots (≥ 1.5 cm long) from the explants were defoliated, decapitated, and horizontally cultured on GD1 medium supplemented with 0.88 μM 6-benzylaminopurine (BAP) and various concentrations of AgNO3. Axillary shoot proliferation was obtained through an 8-wk cyclic culture in which the concentration of BAP gradually decreased. Decapitation and horizontal culture of explants together with the addition of 17.67 μM AgNO3 proved beneficial for shoot proliferation and elongation; however, the efficiency of this method diminished when activated charcoal was added. The elongated shoots were rooted with indole-3-butyric acid (IBA, 123 μM) pulse stimulation for 48 h, after which the shoots were transferred to IBA-free root expression medium with 17.67 μM AgNO3, and they achieved a better rooting percentage (87.5%). Rooted plantlets were acclimatized and successfully grown in a greenhouse, with a survival rate of 72.3%.