Abstract
一、The conservation and application of the genomics and production of secondary products in forestry (3/4)
（一）We have obtained hairy root lines of Taxus sumatrana, Nothopodytes nimmoniana, Camptotheca acuminata , and Cephalotaxus wilsoniana which transformed with rol gene. These root lines grew rapidly and produced stably secondary metabolites greater and more easily to preserve than that from cell cultures. The limitation is that they are difficult to scale up in batch cultures. This study is trying to improve culture techniques including to enhance transformation rates of hairy roots, to increase biomass coupling with high concentration of secondary metabolites, to design suitable bioreactors to grow root cultures, and to establish a cooperative platform among research, application, and biotech companies.（二）For developing the forestry genomic research, the nucleotide sequencing plays the important role. In this project, the specific tree species' genome and transcriptome will be sequencing by using next generation sequencing. The sequencing data will provide useful information for developing molecular marker and improve the studies of the population genetics, molecular breeding and species identification.（三）The preservation and utilization of superior germplasm from in vitro cuLtures (1/4)
In vitro cultures including plantlets, shoot tips, cells, transgenic cells, Androdium cinnamomea strains with special characters will be collected preserved in long term period. All cultures will be tested in viability and productivity of secondary metabolites. We will establish the best condition combing with suitable medium, methods, and temperature to preserve cultures to preserve in long term period.（四）Preservation of fungi native in Taiwan (1/4)
There have 10 special fungi spp. will be studied. Native truffle will be focused in 2019. Kinds of traditional preservations will be tried and estimated.二、Cloning of xylem cellulose synthase gene and breeding of genetically modified trees(5/5)
This 4-year research project is proposed to develop gene-stacking technology for the breeding of new Eucalyptus varieties in which multiple genetic characteristics are improved to meet the demand in industry. Preservation technology is also proposed to develop for the establishment of mutant bank containing varied transgenic lines of Eucalyptus that will bring the molecular breeding into the new research area of functional genomics. In this fourth years project, gene stacking by sequential transformation will be developed to raise transgenic E. camaldulensis plants with the antisense Pt4CL1 and the sense CAld5H transgenes. The transgenic E. camaldulensis plants previously transformed with the sense CAld5H transgenes will be sequentially transferred with recombinant SiRNA of the CAD gene to produced transgenic callus or shoots with double transgenes. In co-transformation, transgenic Eucalyptus plants with the antisense Euc4CL1 and/or the sense CALd5H transgene will be produced. The transgenic E. camaldulensis plants previously transformed with the sense CAld5H transgenes will be selected and transferred with recombinant SiRNA of the CAD gene to produce transgenic callus or shoots with multiple transgenes. In addition to these, another 2 cellulose synthase (CesA) genes will be cloned from E. camaldulensis readily for the further co-transformation work in the following year. In controlled pollination of gene stacking, transgenic seedlings with C4H and GUS transgenes derived from pollination between transgenic E. grandis x E. urophylla and E. camaldulensis will be raised. Transgenic Eucalyptus previously transformed with either the antisense Euc4CL1or the sense CAld5H transgene will be induced to flower for making more controlled pollination. For rapidly measuring monomeric lignol of transgenic wood, an analytical technique by using pyrolyzer coupled with GC-MS will be developed. The low-temperature preservation technique developed in the last year's project will be used to preserve varied transgenic shoots produced from this or previous projects.
三、Research of breeding, Silviculture and Utility for economical tree species:
（一）Improvedd seed production of seed orchard and progeny test of Acacia confusa.（二）Provenancece and progeny test and selection of superior families for A. confusa and Trema orientalis.（三）Trials of Planting densities of A. confusa and Alnus formosana.（四）Fertilization trias of A. confusa.
四、Study on the high efficiency production of scattered bambooThis study tends to investigate the stand structure, growth and biomass of scattered bamboo to understand the status of unmanaged and managed bamboo stand. Develop a method that increases the efficiency of bamboo cutting and skidding in scattered bamboo stands, as the basis for sustainable production and utilization of bamboo resources. In addition, we also want to hold a promotional event to educate the people the importance of sustainable use of bamboo resources."