公告日期 : 2012-05-10
Production of Acteoside in Hairy-Root Culture of Paulownia fortunei Hemsl.
Authors：Kuo-Lung Ku, Ching-Fen Hsu, Yue-Ken Liao
In vitro grown paulownia (Paulownia fortunei Hemsl.) seedlings were inoculated with Agrobacterium rhizogenes (BCRC 15011; (ATCC 43057)) to produce hairy roots for acteoside (AS) production. Hairy-root lines 7-6, 23-1, and 55-1 were first cultured in MS liquid medium (50 mL flask-1) to monitor their AS production, and line 55-1 was determined to have the maximal productivity (13.12±2.36 mg flask-1). Subsequent examinations of this culture line revealed that MS medium and its dilutions (×1/2 and ×1/4 strength) all provided suitable nutrient components for AS production and were better than the other concentrations tested (p < 0.05). It was observed that when the medium was supplemented with 60 g L-1 sucrose, better AS production was also achieved (p < 0.05) due to enhancement of root growth. Ethanol (EtOH; 9.5% (v/v)), a commonly used solvent for elicitor preparation, was determined to be inhibitory and caused a correspondingly low AS content when applied to the medium. A 24-h pulse treatment of methyl jasmonate (MeJA), dissolved in an appropriate concentration of EtOH and adjusted to a final concentration of 0~50 μM, was tested on a 28-d-old root culture and was noted to have had a non-significant effect on AS accumulation. Further investigation is necessary to define a better combination of pulse duration and elicitor concentration to improve AS production with this culture system.